The Quick-DNA Fecal/Soil Microbe Miniprep Kit is a fecal and soil DNA extraction kit designed for the simple and rapid isolation of inhibitor-free, PCR-quality host cell and microbial DNA from a variety of sample sources, including humans, birds, rats, mice, cattle, etc. The procedure is easy and can be completed in minutes: fecal samples are rapidly and efficiently lysed by bead beating with our state of the art, ultra-high density BashingBeads. Zymo-Spin column technology is then used to isolate the DNA, which is subsequently filtered to remove humic acids/polyphenols that can inhibit PCR. Eluted DNA is ideal for downstream molecular-based applications including PCR, arrays, genotyping, methylation detection, etc.
– Rapid method for the isolation of inhibitor-free, PCR-quality DNA from fecal samples in minutes, including from humans, birds, rats, mice, cattle, etc.
– Ultra-high density BashingBeads are fracture resistant and chemically inert.
– Zymo-Spin column and unique filtration technologies effectively removes PCR inhibitors from the DNA product.
| Applicable For | All sensitive downstream applications such as qPCR and Next-Generation Sequencing. |
|---|---|
| Elution Volume | ≥ 50 µl |
| Equipment | Microcentrifuge, vortex, cell disruptor/pulverizer |
| Processing Volume | ≤150 mg feces, ≤ 250 mg soil, ≤100 mg fungal/bacterial cells (wet weight) |
| Purity | A260/A280 nm ≥1.8. |
| Sample Source | Feces or soil |
| Sample Storage | DNA stored at ≤ -20°C. |
| Size Range | Capable of recovering genomic DNA up to and above 40 kb. In most instances, mitochondrial DNA and viral DNA (if present) will also be recovered. |
| Type | Total DNA |
| Yield | ≤ 25 µg total DNA |
Order information
| Cat # | Name | Size |
|---|---|---|
| D6010 | Quick-DNA Fecal/Soil Microbe DNA Miniprep Kit | 50 Preps |
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