ZymoBIOMICS 96 MagBead DNA Kit

The ZymoBIOMICS DNA Kits are microbial DNA purification kits designed for purifying DNA from a variety of sample inputs that is immediately ready for microbiome or metagenome analyses. The ZymoBIOMICS lysis system eliminates bias associated with unequal lysis efficiencies of different organisms (e.g. Gram-negative/positive bacteria, fungus, protozoans, and algae), making it ideal for microbial community profiling. Uniform mechanical lysis of all microbes is achieved by bead beating with the innovative ultra-high density BashingBeads. This kit is equipped with our OneStep PCR Inhibitor removal technology, enabling PCR amplification from DNA derived from inhibitor-rich environmental samples. Purified DNA is ideal for all downstream applications including PCR, arrays, 16S rRNA gene sequencing, and shotgun sequencing. DNA Size is 15-20 kb.

– High-throughput purification of high quality, inhibitor-free DNA from any sample including feces, soil, water, biofilms, swabs, saliva, and body fluids.
– The ZymoBIOMICS innovative lysis system enables efficient and unbiased lysis of microbes including Gram-positive/negative bacteria, fungi, protozoans, algae, etc.
– The automation friendly workflow enables nearly any sample to be processed in as little as 90 minutes for 96 prep.

Applicable ForAll sensitive downstream applications such as qPCR and Next-Generation Sequencing.
Elution Volume≥ 37.5 µl DNA Elution Buffer or DNase free water
EquipmentCentrifuge fitted with a 96 well microplate carrier, 96 Well Magnetic Stand, Liquid handler or other robotic sample processor, 96 well plate heat block
Processing VolumeFecal ≤100 mg, Soil ≤ 200 mg, Cells ≤ 20 mg (Approximately equal to 2×108 bacterial, 2×107 yeast, or 2×106 mammalian cells)
PurityHigh quality, inhibitor-free DNA is eluted with ZymoBIOMICS DNase/RNase Free Water and is suitable for all downstream applications including PCR and Next-Generation sequencing
Sample SourceBacterial, fungal, protozoan, algae, viral, mitochondrial, and host DNA is efficiently isolated from feces, soil, fungal/bacterial cells, biofilms and water.
Sample StorageEluted DNA should be stored at ≤ -20°C.
Size RangeTypically 15-20 kb post-bead beating. For optimal DNA integrity, collect samples in DNA/RNA Shield.
TypeTotal DNA
YieldUp to 10 µg total DNA can be eluted into 50 µl (37.5 µl minimum)

Order information
Cat # Name Size
D4302 ZymoBIOMICS 96 MagBead DNA Kit (Lysis Rack) 2 x 96 Preps
D4306 ZymoBIOMICS 96 MagBead DNA Kit (No Lysis Matrix) 2 x 96 Preps
D4308 ZymoBIOMICS 96 MagBead DNA Kit (Lysis Tubes) 2 x 96 Preps
D4302-E ZymoBIOMICS 96 MagBead DNA Kit – Dx (Lysis Rack) 2 x 96 Preps
D4306-E ZymoBIOMICS 96 MagBead DNA Kit – Dx (No Lysis Matrix) 2 x 96 Preps
D4308-E ZymoBIOMICS 96 MagBead DNA Kit – Dx (Lysis Tubes) 2 x 96 Preps
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