Overview The ZymoBIOMICS Gut Microbiome Standard is an accurately quantified microbial community mimicking the human gut microbiome. The challenges it presents to microbiomics profiling allows researchers to validate protocols and fine-tune them for gut microbiome research and act as a positive control for sample processing. Highlights – True to Life: comprised Read more…
Overview ZymoBIOMICS Spike-in Control II (Low Microbial Load) consists of three bacteria strains, Truepera radiovictrix, Imtechella halotolerans and Allobacillus halotolerans. When spiked into a microbial sample, this product will serve as an in situ positive control for DNA-sequencing-based microbiome measurements. Of the three chosen bacteria, Imtechella halotolerans is Gram-negative and Allobacillus halotolerans is Gram-positive. Truepera radiovictrix is resistant to lysozyme lysis and Read more…
Overview ZymoBIOMICS™ Spike-in Control I (High Microbial Load) consists of equal cell numbers of two bacteria strains, Imtechella halotolerans and Allobacillus halotolerans. When spiked into an unknown sample, this product will serve as an in situ positive control for DNA-sequencing-based (e.g. NGS-based) microbiome measurements. These two bacteria, Imtechella halotolerans (Gram-negative) and Allobacillus halotolerans (Gram-positive) represent different cell recalcitrance and can Read more…
Overview One of the major challenges in the emerging field of microbiomics is the bias and errors introduced in the complex workflows. Besides nucleic acid purification, bias also arises from sequencing library preparation and subsequent processes. The ZymoBIOMICS Microbial Community DNA Standard is designed to assess bias, errors and other Read more…
Overview Microbial composition profiling techniques powered by Next-Generation sequencing are becoming routine in microbiomics and metagenomics studies. However, these analytical techniques can suffer from significant bias from collection to analysis. The ZymoBIOMICS Microbial Community Standard is designed to assess bias and errors in the extraction methods of a microbiomics workflow. Read more…
Overview The Quick-ITS Plus NGS Library Prep Kit is the fastest and simplest NGS library prep targeting the ITS region for high-throughput sequencing. The automation-friendly protocol utilizes a single qPCR/PCR for combined targeted amplification and barcode addition using specially designed primers. After pooling by equal volume, a single clean-up of the Read more…
Overview The Quick-16S Plus NGS Library Prep Kit (V3-V4, UDI) is the fastest and simplest NGS library prep targeting the V3-V4 region of the 16S rRNA gene for high-throughput sequencing. The automation-friendly protocol utilizes a single qPCR/PCR for combined targeted amplification and barcode addition using specially designed primers. After pooling by Read more…
Overview The Quick-16S Plus NGS Library Prep Kit (V4) is the fastest and simplest NGS library prep targeting the V4 region of the 16S rRNA gene for high-throughput sequencing. The automation-friendly protocol utilizes a premixed amplification plate and a single qPCR/PCR for combined targeted amplification and barcode addition. Pooling by equal Read more…
Overview 16S rRNA sequencing is a routine technique for microbiome composition profiling. Compared to shotgun metagenomics sequencing, 16S rRNA sequencing is more cost-effective and more robust; it generally requires less input DNA and is less impacted by the presence of host DNA. However, 16S rRNA sequencing has its own challenges. Read more…
Overview The Femto Bacterial DNA Quantification Kit can be used to detect and quantify bacterial DNA with high specificity and sensitivity. Bacterial DNA can be reliably quantified in a background of non-bacterial DNA such as fungal, animal, and plant DNA, etc. This is essential for downstream applications that require accurate Read more…
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