One of the major challenges in the emerging field of microbiomics is the bias and errors introduced in the complex workflows. Besides nucleic acid purification, bias also arises from sequencing library preparation and subsequent processes. The ZymoBIOMICS Microbial Community DNA Standard is designed to assess bias, errors and other artifacts after the step of nucleic acid purification. This DNA standard is created by pooling DNA extracted from pure cultures. It has an accurately defined composition, negligible impurities (0.01%), and contains genomes of a wide range of GC content (15% – 85%). This DNA standard is designed to have the same microbial composition with the cellular version, the ZymoBIOMICS Microbial Community Standard, so that they can be more powerful when working in tandem. Theoretical Composition Based on Genomic DNA: Listeria monocytogenes – 12%, Pseudomonas aeruginosa – 12%, Bacillus subtilis – 12%, Escherichia coli – 12%, Salmonella enterica – 12%, Lactobacillus fermentum – 12%, Enterococcus faecalis – 12%, Staphylococcus aureus – 12%, Saccharomyces cerevisiae – 2%, and Cryptococcus neoformans – 2%.
– Accurate composition: composition cross-validated with multiple types of measurements.
– Negligible impurity: guaranteed to contain < 0.01% foreign microbial DNA.
– Wide range of GC content: 15%-85%, for assessing bias caused by GC content variation.
| Purity | < 0.01% foreign microbial DNA |
|---|---|
| Sample Source | A mixture of genomic DNA of ten microbial strains. |
| Sample Storage | -20 °C |
Order information
| Cat # | Name | Size |
|---|---|---|
| D6305 | ZymoBIOMICS Microbial Community DNA Standard | 200ng |
| D6306 | ZymoBIOMICS Microbial Community DNA Standard | 2000ng |
Other products
ZymoBIOMICS Gut Microbiome Standard
Overview The ZymoBIOMICS Gut Microbiome Standard is an accurately quantified microbial community mimicking the human gut microbiome. The challenges it presents to microbiomics profiling allows researchers to validate protocols and fine-tune them for gut microbiome
ZymoBIOMICS Spike-in Control II (Low Microbial Load)
Overview ZymoBIOMICS Spike-in Control II (Low Microbial Load) consists of three bacteria strains, Truepera radiovictrix, Imtechella halotolerans and Allobacillus halotolerans. When spiked into a microbial sample, this product will serve as an in situ positive control for DNA-sequencing-based microbiome measurements. Of
ZymoBIOMICS Spike-in Control I (High Microbial Load)
Overview ZymoBIOMICS™ Spike-in Control I (High Microbial Load) consists of equal cell numbers of two bacteria strains, Imtechella halotolerans and Allobacillus halotolerans. When spiked into an unknown sample, this product will serve as an in situ positive control for DNA-sequencing-based (e.g.