ZymoBIOMICS Spike-in Control II (Low Microbial Load) consists of three bacteria strains, Truepera radiovictrix, Imtechella halotolerans and Allobacillus halotolerans. When spiked into a microbial sample, this product will serve as an in situ positive control for DNA-sequencing-based microbiome measurements. Of the three chosen bacteria, Imtechella halotolerans is Gram-negative and Allobacillus halotolerans is Gram-positive. Truepera radiovictrix is resistant to lysozyme lysis and has very high GC content. They represent different challenges in NGS-based analysis. Moreover, with accurately quantified cell number and a log abundance distribution, this standard enables absolute cell number quantification in cases such as pathogen load detection.
– Absolute Quantification: Enables cell number measurements from low bacterial load samples using Next-Gen Sequencing.
– In situ Quality Control: Ensures each sample is quantified accurately.
– Log Abundance Distribution: Three microbes alien to the human microbiome in log-distribution from 103 to 105 cells.
| 16S rRNA genes | https://s3.amazonaws.com/zymo-files/BioPool/D6321.refseq.zip |
|---|---|
| Biosafety | This product is not biohazardous as microbes have been fully inactivated. |
| Impurity Level | < 0.01% foreign microbial DNA |
| Sample Storage | -80°C |
| Storage Solution | DNA/RNA Shield™ |
Order information
| Cat # | Name | Size |
|---|---|---|
| D6321 | ZymoBIOMICS Spike-in Control II (Low Microbial Load) | 25 Preps |
| D6321-10 | ZymoBIOMICS Spike-in Control II (Low Microbial Load) | 250 Preps |
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