High-quality intact small and large RNAs are efficiently recovered using a Direct-zol™ RNA kit compared to using a Supplier Q kit. RNA is DNA-free and ready for all downstream applications including NGS.
RNA purified from TRIzol® using Direct-zol™ RNA compared to an unbiased method (mirVana™, Ambion). Data is highly correlated (2837 overlapped miRNA: r2 = 0.9706). Analysis was performed using miRNA-Seq (MiSeq®, Illumina).
Direct-zol™ RNA kits recovered ~4-fold more miRNAs (< 40 nt) than conventional methods. miRNAs purified from cells and tissue were quantified using Bioanalyzer small RNA chip.
| Product | Cat. No. | Size | |
|---|---|---|---|
| Direct-zol™ RNA Miniprep Kit | R2050 | 50 preps | - |
| Direct-zol™ RNA Miniprep Plus Kit | R2070 | 50 preps | R2070S(10 preps) |
TRIzol® and TRI Reagent® are registered trademarks of Molecular Research Center, Inc.
U.S. Patent No. 9,051,563 B2 and other pending patents. Direct-zol™ is a trademark of Zymo Research Corp.
The Quick-RNA™ Miniprep Plus Kit is universal and accommodates any sample input including cultured cells, any tissue, whole blood, tough-to-lyse samples, etc.
RNA isolated with Quick-RNA™ is DNA-free compared to a Supplier Q kit. Total RNA was isolated from 106 human epithelial cells (with in-column DNase treatment for both kits, n=3).
High-quality RNA was consistently isolated with the Quick-RNA™ Miniprep Plus Kit (left), but not with Supplier Q kits (right). Quality was assessed using the Agilent 2200 TapeStation® system; red = low quality
| Product | Cat. No. | Size |
|---|---|---|
| Quick-RNA™ Miniprep Plus Kit | R1057 | 50 preps |
Based on cited journal articles, 67% of all RNA Clean & Concentrator™ (RCC™) journal citations are used for library preparation, and 52% of the same set of citations are used for Illumina protocols and workflows.
RNA was cleaned-up using the RCC™ kit and a Supplier Q kit (n=4). RNA purity (measured by A260/230) was greater than 1.8 for the RCC™ kit but not for the Supplier Q kit.
Increasing amounts of RNA was cleaned up using the RCC™ kit and a Supplier Q kit (n=2). RCC™ provides higher yields and more consistent recovery when compared to the Supplier Q kit.
| Product | Cat. No. | Size | |
|---|---|---|---|
| RNA Clean & Concentrator™-5 | R1015 | 50 preps | R1015S (5 preps) |
| RNA Clean & Concentrator™-5 w/ DNase I | R1013 | 50 preps | - |
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