DNA and RNA in stool is effectively stabilized in DNA/RNA Shield™ at ambient temperature. Graphs show: spike-in DNA and RNA controls from stool purified at the indicated time points and analyzed by (RT)qPCR.
Viruses, bacteria and yeast are effectively inactivated by DNA/RNA Shield™. Samples containing the infectious agent (virus, bacteria, yeast) were treated for 5 minutes with DNA/RNA Shield™ or mock (PBS). Titer (PFU) was subsequently determined by plaque assay. Validated by: Influenza A – D. Poole and Prof. A. Mehle, Department of Medical Microbiology and Immunology, University of Wisconsin, Madison; Ebola (Kikwit) – L. Avena and Dr. A. Griffiths, Department of Virology and Immunology, Texas Biomedical Research Institute; HSV-1/2 – H. Oh, F. Diaz and Prof. D. Knipe, Virology Program, Harvard Medical School; E. coli, L. fermentum, B. subtilis, S. cerevisiae – Zymo Research).
*Disclaimer: This graph only displays results from E. coli inactivation. Each microbe was tested independently and were combined into one graph for brevity. Bacterial cultures were grown between 108 – 109 cells and yest cultures were grown between 107 – 108 cells.
No Reagent Removal. Simple, direct purification using Zymo Research Purification Products*.
Sample in DNA/RNA Shield
Simply add binding reagent
Bind directly
to spin-column or Magbeads
Eluted DNA/RNA
is ready to use
Product | Cat. No. | Size | |
---|---|---|---|
DNA/RNA Shield™ | R1100-50 | 50ml | R1100-8-S (8 ml) |
DNA/RNA Shield™ (2X Concentrate) | R1200-25 | 25ml | - |
Evacuated blood tube (16 x 100 mm) filled with 6 ml of DNA/RNA Shield™
for the direct collection of 3 ml whole-blood (human or animal).
15 ml container (prefilled with 9 ml DNA/RNA Shield™) for the direct collection of up to 1 gram and/or 1 ml stool. Collection spoon is included in the container screwcap.
Microbe & Tissue Lysis Tubes
2 ml collection tube (prefilled with 1 ml DNA/RNA Shield™) and BashingBeads™ for the collection and homogenization of microbial or tissue samples.
5 ml screwcap container (12 x 80 mm) filled with 1 ml DNA/RNA Shield™
and a sterile swab for specimen collection.
Product | Cat. No. | Size | |
---|---|---|---|
DNA/RNA Shield™ - Blood Collection Tube | R1150 | 50 pack | - |
DNA/RNA Shield™ - Fecal Collection Tube | R1101 | 10 pack | - |
NA/RNA Shield™ Lysis Tube (Microbe) | R1103 | 50 pack | - |
DNA/RNA Shield™ Lysis Tube w/ Swab (Microbe) | R1104 | 50 pack | - |
DNA/RNA Shield™ Lysis Tube (Tissue) | R1105 | 50 pack | - |
DNA/RNA Shield™ - Swab & Collection Tube | R1106 R1107 |
10pack (1 ml fill) 50 pack (1 ml fill) |
R1106S (10 pack) |
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