Overview Microbial composition profiling techniques powered by Next-Generation sequencing are becoming routine in microbiomics and metagenomics studies. However, these analytical techniques can suffer from significant bias from collection to analysis. The ZymoBIOMICS Microbial Community Standard is designed to assess bias and errors in the extraction methods of a microbiomics workflow. Đọc tiếp…
Overview One of the major challenges in the emerging field of microbiomics is the bias and errors introduced in the complex workflows. Besides nucleic acid purification, bias also arises from sequencing library preparation and subsequent processes. The ZymoBIOMICS Microbial Community DNA Standard is designed to assess bias, errors and other Đọc tiếp…
Overview ZymoBIOMICS™ Spike-in Control I (High Microbial Load) consists of equal cell numbers of two bacteria strains, Imtechella halotolerans and Allobacillus halotolerans. When spiked into an unknown sample, this product will serve as an in situ positive control for DNA-sequencing-based (e.g. NGS-based) microbiome measurements. These two bacteria, Imtechella halotolerans (Gram-negative) and Allobacillus halotolerans (Gram-positive) represent different cell recalcitrance and can Đọc tiếp…
Overview 16S rRNA sequencing is a routine technique for microbiome composition profiling. Compared to shotgun metagenomics sequencing, 16S rRNA sequencing is more cost-effective and more robust; it generally requires less input DNA and is less impacted by the presence of host DNA. However, 16S rRNA sequencing has its own challenges. Đọc tiếp…
Overview The Quick-16S Plus NGS Library Prep Kit (V4) is the fastest and simplest NGS library prep targeting the V4 region of the 16S rRNA gene for high-throughput sequencing. The automation-friendly protocol utilizes a premixed amplification plate and a single qPCR/PCR for combined targeted amplification and barcode addition. Pooling by equal Đọc tiếp…
Overview The Quick-16S Plus NGS Library Prep Kit (V3-V4, UDI) is the fastest and simplest NGS library prep targeting the V3-V4 region of the 16S rRNA gene for high-throughput sequencing. The automation-friendly protocol utilizes a single qPCR/PCR for combined targeted amplification and barcode addition using specially designed primers. After pooling by Đọc tiếp…
Overview The HostZERO Microbial DNA Kit is designed to overcome the challenge of contaminating host nucleic acids in microbial samples. This kit uses a novel method to reduce the amount of contaminating host DNA by selectively lysing the eukaryotic cells and degrading this DNA prior to total DNA purification. Paired Đọc tiếp…
Overview The Quick-DNA Fecal/Soil Microbe Miniprep Kit is a fecal and soil DNA extraction kit designed for the simple and rapid isolation of inhibitor-free, PCR-quality host cell and microbial DNA from a variety of sample sources, including humans, birds, rats, mice, cattle, etc. The procedure is easy and can be completed Đọc tiếp…
Overview The Select-a-Size DNA MagBead Kit is a size selection DNA magbead kit that provides the fastest and easiest method to purify specific ranges of DNA fragments from PCR, endonuclease digestions, ligations, library preparations, adapter removal, etc. This simple workflow allows for specific cutoffs that can be modified to suit Đọc tiếp…
Overview The ZymoBIOMICS DNA Kits are microbial DNA purification kits designed for purifying DNA from a variety of sample inputs that is immediately ready for microbiome or metagenome analyses. The ZymoBIOMICS lysis system eliminates bias associated with unequal lysis efficiencies of different organisms (e.g. Gram-negative/positive bacteria, fungus, protozoans, and algae), Đọc tiếp…
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