ZymoBIOMICS Spike-in Control I (High Microbial Load)

ZymoBIOMICS™ Spike-in Control I (High Microbial Load) consists of equal cell numbers of two bacteria strains, Imtechella halotolerans and Allobacillus halotolerans. When spiked into an unknown sample, this product will serve as an in situ positive control for DNA-sequencing-based (e.g. NGS-based) microbiome measurements. These two bacteria, Imtechella halotolerans (Gram-negative) and Allobacillus halotolerans (Gram-positive) represent different cell recalcitrance and can expose potential bias during DNA extraction. Moreover, with accurately quantified cell numbers, this standard enables absolute cell number quantification in microbiome measurements.

– Absolute Quantification: enables absolute cell number measurements using Next-Generation Sequencing
– in situ Quality Control: ensures each sample is quantified accurately
– Unique composition: comprised of two microbes alien to the human microbiom.

BiosafetyThis product is not biohazardous as microbes have been fully inactivated.
Impurity Level< 0.01% foreign microbial DNA
Reference GenomesComplete reference genomes and 16S rRNA genes
Sample Storage-80°C
Storage SolutionDNA/RNA Shield

Order information
Cat #NameSize
D6320ZymoBIOMICS Spike-in Control I (High Microbial Load)25 Preps
D6320-10ZymoBIOMICS Spike-in Control I (High Microbial Load)250 Preps
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ZymoBIOMICS Spike-in Control I (High Microbial Load)

Overview ZymoBIOMICS™ Spike-in Control I (High Microbial Load) consists of equal cell numbers of two bacteria strains, Imtechella halotolerans and Allobacillus halotolerans. When spiked into an unknown sample, this product will serve as an in situ positive control for DNA-sequencing-based (e.g.

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ZymoBIOMICS Microbial Community Standard

Overview Microbial composition profiling techniques powered by Next-Generation sequencing are becoming routine in microbiomics and metagenomics studies. However, these analytical techniques can suffer from significant bias from collection to analysis. The ZymoBIOMICS Microbial Community Standard

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ZymoBIOMICS Spike-in Control II (Low Microbial Load)

Overview ZymoBIOMICS Spike-in Control II (Low Microbial Load) consists of three bacteria strains, Truepera radiovictrix, Imtechella halotolerans and Allobacillus halotolerans. When spiked into a microbial sample, this product will serve as an in situ positive control for DNA-sequencing-based microbiome measurements. Of

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